Guide to molecular cloning techniques /
Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA...
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| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Orlando :
Academic Press,
1987.
|
| Series: | Methods in enzymology ;
v. 152. |
| Subjects: |
MARC
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| 245 | 0 | 0 | |a Guide to molecular cloning techniques / |c edited by Shelby L. Berger, Alan R. Kimmel. |
| 260 | |a Orlando : |b Academic Press, |c 1987. | ||
| 300 | |a xl, 812 pages : |b illustrations ; |c 24 cm. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a unmediated |b n |2 rdamedia | ||
| 338 | |a volume |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v v. 152 | |
| 540 | |a Current copyright fee: GBP32.84 |c 5\1. |5 Uk | ||
| 520 | |a Requirements for a molecular biology laboratory. General methods for isolating and characterizing nucleic acids. Enzymatic techniques and recombinant DNA technology. Restriction enzymes. Growth and maintenance of bacteria. Genomic cloning. Preparation and characterization of RNA. Preparation of cDNA and the generation of cDNA libraries. Selection of clones from libraries. Identification and characterization of specific clones. | ||
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Section I. Requirements for a molecular biology laboratory -- Section II. General methods for isolating and characterizing nucleic acids -- Section III. Enzymatic techniques and recombinant DNA technology -- Section IV. Restriction enzymes -- Section V. Growth and maintenance of bacteria -- Section VI. Genomic cloning -- Section VII. Preparation and characterization of RNA -- Section VIII. Preparation of cDNA and the generation of cDNA libraries -- Section IX. Selection of clones from libraries -- Section X. Identification and characterization of specific clones. | |
| 650 | 0 | |a Molecular cloning. | |
| 650 | 0 | |a Recombinant DNA. | |
| 650 | 0 | |a Molecular cloning |x Technique. | |
| 650 | 0 | |a Cloning |x Technique. | |
| 650 | 0 | |a DNA. | |
| 650 | 2 | |a Cloning, Molecular |x methods |0 (DNLM)D003001Q000379 | |
| 650 | 2 | |a DNA, Recombinant |0 (DNLM)D004274 | |
| 650 | 2 | |a Cloning, Molecular |0 (DNLM)D003001 | |
| 650 | 2 | |a DNA |0 (DNLM)D004247 | |
| 650 | 6 | |a Clonage moléculaire |0 (CaQQLa)201-0032258 |x Technique. |0 (CaQQLa)201-0377564 | |
| 650 | 6 | |a Clonage |0 (CaQQLa)201-0038611 |x Technique. |0 (CaQQLa)201-0377564 | |
| 650 | 6 | |a ADN. |0 (CaQQLa)201-0008510 | |
| 650 | 6 | |a Clonage moléculaire. |0 (CaQQLa)201-0032258 | |
| 650 | 6 | |a ADN recombinant. |0 (CaQQLa)201-0024828 | |
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| 650 | 7 | |a Cloning. |2 cabt | |
| 650 | 7 | |a Clonage. |2 eclas | |
| 650 | 7 | |a ADN = Acide désoxyribonucléique. |2 eclas | |
| 650 | 7 | |a Génétique. |2 eclas | |
| 650 | 7 | |a Manuels. |2 eclas | |
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| 650 | 7 | |a Klonierung |2 gnd |0 (DE-588)4192480-0 | |
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| 653 | |a Biologia molecular | ||
| 653 | |a Laboratorio | ||
| 653 | |a Acido nucleico | ||
| 653 | |a DNA recombinante | ||
| 653 | |a Bacteria | ||
| 653 | |a Clonagem | ||
| 653 | |a RNA | ||
| 653 | |a Molecular biology | ||
| 653 | |a Laboratory | ||
| 653 | |a Nucleic acids | ||
| 653 | |a Recombinant DNA | ||
| 653 | |a Cloning | ||
| 653 | |a Clones | ||
| 653 | 0 | 0 | |a genetische modificatie |
| 653 | 0 | 0 | |a genetic engineering |
| 653 | 0 | 0 | |a handleidingen |
| 653 | 0 | 0 | |a guide books |
| 653 | 0 | 0 | |a dierkloneren |
| 653 | 0 | 0 | |a animal cloning |
| 653 | 1 | 0 | |a Molecular Biology (General) |
| 653 | 2 | 0 | |a Molecular Genetics |
| 653 | 2 | 0 | |a Proteins and Enzymes |
| 653 | 1 | 0 | |a Moleculaire biologie (algemeen) |
| 653 | 2 | 0 | |a Moleculaire genetica |
| 653 | 2 | 0 | |a Eiwitten en enzymen |
| 655 | 4 | |a Aufsatzsammlung. | |
| 655 | 4 | |a RNS-Klonierung. | |
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| 655 | 7 | |a Textbooks (form) |0 (NL-LeOCL)088143252 |2 gtt | |
| 700 | 1 | |a Berger, Shelby L., |e editor. | |
| 700 | 1 | |a Kimmel, Alan R., |e editor. | |
| 880 | 0 | |6 505-00 |a Equipping the laboratory / Daphne D. Blumberg -- Creating a ribonuclease-free environment / Daphne D. Blumberg -- Safety with 32P- and 35S-labeled compounds / Robert A. Zoon -- Large- and small-scale phenol extractions / Donald M. Wallace -- Precipitation of nucleic acids / Donald M. Wallace -- Quantifying 32P-labeled and unlabeled nucleic acids / Shelby L. Berger -- Autoradiograms: 35S and 32P / William M. Bonner -- Electrophoresis in agarose and acrylamide gels / Richard C. Ogden, Deborah A. Adams -- Nick translation / Judy Meinkoth, Geoffrey M. Wahl -- Enzymes for modifying and labeling DNA and RNA / Fabio Cobianchi, Samuel H. Wilson -- Properties and uses of restriction endonucleases / Joan E. Brooks -- Restriction and modification in vivo by Escherichia coli K12 / Elisabeth A. Raleigh -- Practical aspects of preparing phage and plasmid DNA: Growth, maintenance, and storage of bacteria and bacteriophage / Harvey Miller -- Host strains that alleviate underrepresentation of specific sequences: Overview / Arlene R. Wyman, Kenneth F. Wertman -- Isolation of genomic DNA / Bernhard G. Herrmann, Anna-Maria Frischauf -- Digestion of DNA: Size fractionation / Anna-Maria Frischauf -- Construction and characterization of a genomic library in λ / Anna-Maria Frischauf -- Cloning large segments of genomic DNA using cosmid vectors / Anthony G. DiLella, Savlo L.C. Woo -- Preparation and characterization of RNA: Overview / Shelby L. Berger -- Isolation of RNA using guanidinium salts / Raymond J. MacDonald, Galvin H. Swift, Alan E. Przybyla, John M. Chirgwin -- Isolation of cytoplasmic RNA: Ribonucleoside-vanadyl complexes / Shelby L. Berger -- Isolation and analysis of nuclear RNA / Joseph R. Nevins -- Isolation of messenger RNA from membrane-bound polysomes / Bernard M. Mechler -- Use of antibodies to obtain specific polysomes / Dennis C. Lynch -- Purification and fractionation of poly(A)+ RNA / Allan Jacobson -- Determination of the molar concentration of messenger RNA / Marc S. Krug, Shelby L. Berger -- Translation in cell-free systems / Rosemary Jagus -- Microinjection into Xenopus Oocytes: Equipment / Michael J.M. Hitchcock, Edward I. Ginns, Carol J. Marcus-Sekura -- Preparation of oocytes for microinjection of RNA and DNA / Carol J. Marcus-Sekura, Michael J.M. Hitchcock -- Translation of messenger RNA in injected frog oocytes / Douglas A. Melton -- Characterization of in vitro translation products / Rosemary Jagus -- Preparation of cDNA and the generation of cDNA libraries: Overview / Alan R. Kimmel, Shelby L. Berger -- First-strand cDNA synthesis primed with oligo(dT) / Marc S. Krug, Shelby L. Berger -- Second-strand cDNA synthesis: Classical method / Ueli Gubler -- Second-strand cDNA synthesis: mRNA fragments as primers / Ueli Gubler -- Purification of large double-stranded cDNA fragments / William H. Eschenfeldt, Shelby L. Berger -- Hornopolymeric tailing / William H. Eschenfeldt, Robert S. Puskas, Shelby L. Berger -- Adaptors, linkers, and methylation / Ray Wu, Tiyun Wu, Anuradha Ray -- Directional cDNA cloning in plasmid vectors by sequential addition of oligonucleotide linkers / David M. Helfman, John C. Fiddes, Douglas Hanahan -- Cloning cDNA into λgt10 and λgt11 / Jerry Jendrisak, Richard A. Young, James Douglas Engel -- Full-Length cDNA clones: Vector-primed cDNA synthesis / Prescott L. Deininger -- Selection of clones from libraries: Overview / Alan R. Kimmel -- Molecular hybridization of immobilized nucleic acids: Theoretical concepts and practical considerations / Geoffrey M. Wahl, Shelby L. Berger, Alan R. Kimmel -- Amplification, storage, and replication of libraries / Gabriel Vogeli, Paul S. Kaytes -- Screening colonies or plaques with radioactive nucleic acid probes / Geoffrey M. Wahl, Shelby L. Berger -- Isolation of differentially expressed genes / Thomas D. Sargent -- Oligonucleotide probes for the screening of recombinant DNA libraries / R. Bruce Wallace, C. Garrett Miyada -- Gene cloning based on long oligonucleotide probes / William I. Wood -- Hybridization of genomic DNA to oligonucleotide probes in the presence of tetramethylammonium chloride / Anthony G. Dilella, Savio L.C. Woo -- Use of antibodies to screen cDNA expression libraries prepared in plasmid vectors / David M. Helfman, Stephen H. Hughes -- Gene isolation by screening λgt11 libraries with antibodies / Robert C. Mierendorf, Chris Percy, Richard A. Young -- Gene isolation by retroviral tagging / Stephen P. Goff -- Isolation of genes by complementation in yeast / Mark D. Rose -- Identification and characterization of specific clones: Strategy for confirming the validity of presumptive clones / Alan R. Kimmel -- Subcloning / Jonathan R. Greene, Leonard Guarente -- DNA sequencing: Chemical methods / Barbara J.B. Ambrose, Reynaldo C. Pless -- sequencing DNA with dideoxyribonucleotides as chain terminators: Hints and strategies for big projects / Wayne M. Barnes -- Direct sequencing of denatured plasmid DNA / Robert C. Mierendorf, Diana Pfeffer -- Sequencing of RNA transcripts synthesized in vitro from plasmids containing bacteriophage promoters / Robert C. Mierendorf, Diana Pfeffer -- Hybrid selection of mRNA and hybrid arrest of translation / Rosemary Jagus -- Northern and southern blots / Geoffrey M. Wahl, Judy L. Meinkoth, Alan R. Kimmel -- Fast blots: Immobilization of DNA and RNA from cells / Carl Costanzi, David Gillespie -- Identification of genomic sequences corresponding to cDNA clones / Nikolaus A. Spoerel, Fotis C. Kafatos -- Isolation of full-length genes: Walking the chromosome / Nikolaus A. Spoerel, Fotis C. Kafatos -- Cosmid vectors for genomic walking and rapid restriction mapping / Glen A. Evans, Geoffrey M. Wahl -- Mapping of gene transcripts by nuclease protection assays and cDNA primer extension / Frank J. Calzone, Roy J. Britten, Eric H. Davidson -- A molecular titration assay to measure transcript prevalence levels / James J. Lee, Nancy A. Costlow -- Demonstration of tissue-specific gene expression by in Situ hybridization / Lynne M. Angerer, Kathleen H. Cox, Robert C. Angerer -- Expression, identification, and characterization of recombinant gene products in Escherichia coli / Allan R. Shatzman, Martin Rosenberg -- Heterologous gene expression in yeast / Grant A. Bitter -- Use of eukaryotic expression technology in the functional analysis of cloned genes / Bryan R. Cullen -- Identification of regulatory elements of cloned genes with functional assays / Nadia Rosenthal -- Interaction of protein with DNA in vitro / Lothar Hennighausen, Henry K. Lubon -- In Vivo footprinting of specific protein-DNA interactions / P. David Jackson, Gary Felsenfeld. | |
| 830 | 0 | |a Methods in enzymology ; |v v. 152. |x 0076-6879 | |
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