Chemical reagents for protein modification /

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Bibliographic Details
Main Author: Lundblad, Roger L.
Format: Book
Language:English
Published: Boca Raton, Fla. : CRC Press, c2005.
Edition:3rd ed.
Subjects:
Table of Contents:
  • The site-specific chemical modification of proteins.
  • The modification of amino groups.
  • The modification of histidine residues.
  • The modification of arginine.
  • The modification of carboxyl groups.
  • The modification of cysteine.
  • The modification of Cystine.
  • Appendix I: Microprocedure for the reduction and carboxymethylation of proteins.
  • The modification of methionine.
  • The modification of tyrosine.
  • The chemical modification of tryptophan.
  • The chemical cleavage of peptide bonds.
  • The chemical cross-linking of peptide chains.
  • Some reagents used in the modification of proteins.
  • Assay of protein concentration in solution. Microplate biuret assay ; BCA (Bicinchonimic acid) assay for protein ; Dye-binding assay for protein using coomassie brillant ; Amino acid analysis.