Chemical reagents for protein modification /
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Main Author: | |
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Format: | Book |
Language: | English |
Published: |
Boca Raton, Fla. :
CRC Press,
c2005.
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Edition: | 3rd ed. |
Subjects: |
Table of Contents:
- The site-specific chemical modification of proteins.
- The modification of amino groups.
- The modification of histidine residues.
- The modification of arginine.
- The modification of carboxyl groups.
- The modification of cysteine.
- The modification of Cystine.
- Appendix I: Microprocedure for the reduction and carboxymethylation of proteins.
- The modification of methionine.
- The modification of tyrosine.
- The chemical modification of tryptophan.
- The chemical cleavage of peptide bonds.
- The chemical cross-linking of peptide chains.
- Some reagents used in the modification of proteins.
- Assay of protein concentration in solution. Microplate biuret assay ; BCA (Bicinchonimic acid) assay for protein ; Dye-binding assay for protein using coomassie brillant ; Amino acid analysis.